Quantitative disclosure of DNA knot chirality by high-resolution 2D-gel electrophoresis

2D Gel Electrophoresis - An Overview

Modified gel electrophoresis for higher resolution of DNA fingerprints.

Pig-) The amount of polymorphic frag| merits in a DNA fingerprint d e ^ |-f I 1» pends on characteristics of the 0.8* individual's genome, restriction enzymes and minisatellite B [U l I probes used. In addition h ow0-8* i.»% ever, a high resolution of the DNA fragments during gel electrophoresis is essential. Therefore the following simple method was applied: Instead of using an agarose gel of ...

Segmentation of 2d-gel Electrophoresis Images

Introduction Two-Dimensional Gel Electrophoresis technique is a convenient and well-established method to separate thousands of proteins on polyacrylamide gels, according to the differences in their net charge and their molecular mass [1]. Its digital output is an image which depicts proteins as bright or dark spots over a noisy and inhomogeneous background. Each protein is characterized by its...

Geometric Alignment of 2D Gel Electrophoresis Images

OBJECTIVES 2D gel electrophoresis (2-DE) is the method of choice for analyzing protein expression in the field of proteomics, for example, comparing a reference with a test population. However, due to complex physical and chemical processes the locations of proteins generally vary in different 2-DE images. To cope with these variations, accurate geometric alignment of 2-DE images is important. ...

Assessment of DNA damage induced by butachlor on Cyprinuscarpio(L. 1758) using Single Cell Gel Electrophoresis

Butachlor (N-(butoxymethyl)-2-chloro-N-2, 6-diethylacetanillide) is widely used chloroacetanilide herbicide in Asian region, especially in the north of Iran. Several studies have been reported on toxicity of butachlor, but little information is accessible on DNA damaging potential. Thus, the present study was undertaken to investigate of DNA damage by butachlor in Cyprinuscarpio(Pisces: Cyprini...